High order W02-reactive stable oligomers of amyloid-β are produced in vivo and in vitro via dialysis and filtration of synthetic amyloid-β monomer

E Robb; K Perez; LW Hung; CL Masters; KJ Barnham; RA Cherny; AI Bush; PA Adlard; DI Finkelstein

Journal article

High order W02-reactive stable oligomers of amyloid-β are produced in vivo and in vitro via dialysis and filtration of synthetic amyloid-β monomer

E Robb, K Perez, LW Hung, CL Masters, KJ Barnham, RA Cherny, AI Bush, PA Adlard, DI Finkelstein

Journal of Alzheimer S Disease | IOS PRESS | Published : 2015

DOI: 10.3233/JAD-132024

Abstract

Oligomeric forms of amyloid-β (Aβ) are thought to be responsible for the pathogenesis of Alzheimer's disease. While many oligomers of Aβ are thought to be naturally occurring in the brain of humans and/or transgenic animals, it is well known that Aβ oligomers are also readily produced in vitro in the laboratory. In recent studies, we discovered that synthetic monomeric Aβ (4.7 kDa) could be transformed by microdialysis to higher molecular weight species (approximately 56 kDa, by western blot). Surface-enhanced laser desorption/ionization mass spectrometry and electron microscopy further identified these species' as potential Aβ oligomers. The production of similar species could also be produ..

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University of Melbourne Researchers

Kevin Barnham Author

Ashley Bush Author

Paul Adlard Author

David Finkelstein Author

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Grants

Funding Acknowledgements

Australian National Health and Medical Research Council. PAA and DIF are supported by funds from the NH&MRC, the ARC, the Alzheimer's Association (USA), and the Joan and Peter Clemenger Trust. The Florey Institute of Neuroscience and Mental Health acknowledge the strong support from the Victorian Government and in particular the funding from the Operational Infrastructure Support Grant.